Molecular Biology Tools & Techniques Self-Learning Training Program with Mentor Support

From DNA extraction to CRISPR - master the molecular biology techniques that drive modern life sciences.

Not just theory - Learn how molecular biology experiments actually work!

Step by step, at your own pace, with expert mentor support - Get Certificate!

ADMISSIONS OPEN

The Molecular Biology Tools & Techniques – Self-Learning Training Program is a comprehensive, structured course designed to build strong conceptual understanding and practical awareness of essential molecular biology techniques used in research labs, diagnostics, genomics, and biotechnology industries.

This program bridges the gap between theory and real laboratory workflows, enabling learners to understand why, how, and when specific techniques are used—while learning at their own pace, supported by expert mentors.

About the Program

• Program Type: Self-Learning (Online)
• Mode: Recorded Lectures + Experiment Walkthroughs
• Course Access Duration: 40 Days
• Mentor Support: (Doubt Solving, guidance, feedback)
• Eligibility: Life Science / Biotechnology / Microbiology / Biochemistry background (students & professionals)
• Certification: Yes
• Access: Online learning portal

The Molecular Biology Tools & Techniques Self Learning Program offers a structured, step-by-step learning journey covering essential experimental techniques, analytical methods, and modern molecular biology tools.

You learn through:

• Curated self-learning modules
• Recorded experiment walkthroughs
• Hands-on data interpretation
• Assignments & real experimental logic

Dedicated mentor support for doubt-clearing and guidance

Who Should Register?

• B.Sc / M.Sc students in Life Sciences, Biotechnology, Microbiology, Biochemistry
• Fresh graduates preparing for research or industry roles
• PhD aspirants strengthening lab fundamentals
• Working professionals revising molecular biology techniques
• Learners planning careers in genomics, bioinformatics, diagnostics, or R&D
  

Detailed Curriculum

1. Fundamentals of Molecular Biology: Techniques and Analytical Calculations

• Importance of molecular biology techniques
  

• Basic and advanced molecular biology techniques
  

• Analytical calculations:
  

• Concentrations

• Dilutions
  

• Basic units

• Unit conversions

2. Experiment Walkthrough: Calculations & Experimental Design

• Explanation of calculations with worked examples
  

• Designing experiments:
  

• DNA extraction
  

• Protein isolation
  

• Serial dilution principles and calculations
  

3. DNA Extraction and Characterization via Agarose Gel Electrophoresis

• DNA isolation from bacteria
  

• Agarose gel electrophoresis:
  

• Working principle
  

• Gel staining
  

• Band analysis and interpretation
  

4. Experiment Walkthrough: Agarose Gel Electrophoresis

• Preparation of agarose gel
  
• Buffer preparation
  
• Loading dye preparation
• Electrophoresis setup
• Tips to minimize handling and loading errors
  

5. Protein Isolation & Analysis using SDS-PAGE

• Protein isolation from:
  

• Bacteria
  

• Yeast
  

• Plant
  

• Animal (any one source)
  
  

• SDS-PAGE:
  

• Working principle
  

• Gel staining
  

• Protein band analysis
  

6. Experiment Walkthrough: SDS-PAGE

• Preparation of reagents and buffers
  
• Stacking gel and resolving gel preparation
  
• Loading dye preparation
  
• SDS-PAGE setup
  
• Troubleshooting and error minimization
  

7. Introduction to Mass Spectrometry in Biomolecular Analysis

• Basic principles of mass spectrometry
  
• Components of a mass spectrometer
  
• Workflow and steps involved
• Types of mass spectrometry
  
• Protein identification and characterization using MS
  

8. Quantitative & Qualitative Analysis of DNA and Proteins using UV–Visible Spectroscopy

• DNA concentration and purity analysis
  
• Protein concentration and purity analysis
  
• UV–Visible spectroscopy:
  
  

• • Principle
    

  • Instrumentation
    

  • Working
    

  • Applications
    

9. Experiment Walkthrough: UV–Visible Spectroscopy

• Preparation of DNA and protein samples
  
• Buffer preparation
  
• UV–Vis instrument settings
  
• Absorbance (OD) analysis and interpretation
  

10. Polymerase Chain Reaction (PCR): Principles and Applications

• Principle of PCR
  
• Types of PCR
  
• Working mechanism of basic PCR

11. Experiment Walkthrough: PCR

• Preparation of DNA template, Taq polymerase, and buffers
  
• PCR temperature and cycle settings
  
• Analysis of PCR products using gel electrophoresis
  
• Key experimental considerations
  
• Amplicon number calculation
  

12. Primer Design for PCR

• Primer design basics
  
• Key primer design parameters
  
• Primer design tools
  
• Manual melting temperature (Tm) calculation
  

13. Sequence Alignment: Types, Methods & Tools

• Basics of sequence alignment
• Types of alignment
• Local vs global alignment
• Alignment algorithms and computational methods
• Sequence alignment tools:
  
  

  • Overview
    

  • Comparison
    

• Applications of sequence alignment
  
• Hands-on sequence alignment and result interpretation
  

14. Molecular Markers: Classification and Applications

• Hybridization-based markers (RFLP)
  
• PCR-based markers:
  
  

• • RAPD
    

  • AFLP
    

  • SNP
    

  • VNTR
    

• Applications of molecular markers
  

15. Hybridization Techniques: Principles and Applications

• Southern blotting:
  

• Principle
  

• Process

• Applications
  

• Northern blotting:
  

• Principle
  

• Process
  

• Applications
  

• Western blotting:
  

• Principle
  

• Process
  

• Applications
  

16. Experiment Walkthrough: Blotting Techniques

• Preparation of blotting gels
  
• Setup for Southern, Northern, and Western blotting
  
• Buffer preparation
  
• Antibody preparation for Western blot
  
• Analysis and interpretation of blots
  

17. DNA Microarray Technology: Gene Expression & Genotyping

• Principle of DNA microarray
  
• Types of microarrays
  
• Gene expression analysis using microarrays
  
• Applications
  

18. Experiment Walkthrough: DNA Microarray

• Sample preparation
  
• Components of a DNA microarray
  
• Reading and interpreting microarray results
  

19. DNA Sequencing Technologies: From Sanger to NGS

• Basic steps in DNA sequencing
  
• Sanger sequencing (automated)
  
• Next-generation sequencing platforms:
  
  

• • Illumina sequencing by synthesis
    

  • Ion Torrent sequencing
    

  • Nanopore sequencing
    

  • PacBio (third-generation sequencing)
    

20. NGS Data Processing Pipeline

• Raw data quality control
  
• FASTQ format and Phred scores
  
• Read alignment
  
• Variant calling and annotation
  
• Error correction
  
• Challenges in long-read sequencing
  

21. Principles and Applications of Molecular Cloning

• Major steps involved in molecular cloning
  
• Applications of molecular cloning
  

22. Experiment Walkthrough: Molecular Cloning

• Cloning workflow overview
  
• Restriction enzyme digestion
  
• Gel electrophoresis
  
• Ligation reaction
  
• Bacterial transformation
  
• Colony screening
  

23. Bioinformatics Tools for Cloning

• Plasmid mapping using SnapGene
  
• Restriction site analysis using NEBcutter
  
• In-silico cloning using Benchling
  

24. Gene Transfer Techniques: Concepts and Applications

• Physical methods
  
• Chemical methods
  
• Biological methods
  

25. Experiment Walkthrough: Gene Transfer Techniques

• Bacterial transformation
  
• Eukaryotic transfection
  
• Electroporation
  
• Microinjection
  
• Gene gun (biolistics)
  
• Chemicals, buffers, voltages, and experimental parameters
  
• Comparison of bacterial-ready vs eukaryotic-ready cells
  

26. CRISPR–Cas9: Mechanism and Genome Editing Applications

• Origin of CRISPR-Cas9
  
• Structure and components
  
• Genome editing mechanism
  
• DNA repair mechanisms:
  
  

• • NHEJ
    

  • HDR
    

• Applications

• Limitations and strategies to overcome them

Mentor Support – What Makes This Program Different

You’re not learning alone.

✔ Access to experienced mentors
✔ Concept clarification & technical guidance
✔ Assignment feedback
✔ Experimental logic explanation
 ✔ Career and next-step advice

Key Outcomes

By the end of this program, you will be able to:

✔ Understand and explain core molecular biology techniques
✔ Interpret experimental data confidently
✔ Connect wet-lab experiments with downstream applications
✔ Prepare for research projects, internships, and lab roles
✔ Build a strong foundation for advanced genomics & bioinformatics training

Certification

Receive a Certificate of Completion recognizing your training in Molecular Biology Tools & Techniques.

Why This Program Matters?

Modern life science careers demand more than textbook knowledge. This program is designed to help you think like a molecular biologist, understanding experimental design, data interpretation, and decision-making behind every technique.

Whether you are preparing for higher studies, research roles, or biotech and diagnostics careers, this training equips you with the conceptual depth and practical awareness expected in real-world laboratories.

Ready to Get Started?

Admissions Open

Take the next step toward strengthening your molecular biology foundation with a self-paced, mentor-supported learning experience.

Enroll now and start learning today.