Mass Spectrometry Based Proteomics Analysis Certification Course
Mass spectrometry (MS) is an advanced analytical technique for accurate mass measurement. It does so by producing charged molecular species in vacuum followed by its separation in the magnetic & electric fields on the basis of their mass to charge (m/z) ratio.
Mass spectrometry-based proteomics is emerging as a broadly effective means for identification, characterization & quantification of proteins that are integral components of the various processes essential for life. The characterization of proteins at the proteome & sub-proteome (e.g., the phosphoproteome, proteoglycan, or degradome/peptidome) levels provides a foundation for understanding the fundamental aspects of biology. And amongst the toolkit of techniques with which proteins can be investigated on a large scale, mass spectrometry (MS) has gained popularity because of its ability to handle the complexities associated with the proteome. Other techniques such as two-dimensional gel electrophoresis (2DE), two-hybrid analysis, and protein microarrays fail to achieve the depth of informative proteome analysis seen with mass spectrometry.
The 3 primary applications of Mass Spectrometry to proteomics are cataloging protein expression, defining protein interactions, and identifying sites of protein modification. The use of Mass Spectrometry for proteomics is not just the application of a single technique for all purposes but rather a collection of methodologies, each with strengths suited to particular inquiries.
Mass Spectrometry-based proteomics increasingly contributes to our understanding of the dynamics, interactions, and roles that proteins & peptides play, advancing our understanding of biology and its concepts on a systems-wide level for a wide range of applications including the investigations of microbial communities, bioremediation, and human health.
Importance/ Benefits of the Mass Spectrometry-Based Proteomics Analysis Certification Course:
The main significance of proteomics research is still studying the phenomenon of life and promoting human health. Proteins are the executors of physiological functions and the direct embodiment of life. The study of protein structure & function will directly clarify the mechanism of life under physiological or say pathological conditions. Some phenomena of life is not only determined by a certain protein, but also the interaction of multiple proteins that requires the study of proteomics.
Performing mass spectrometry-based proteomics requires background knowledge and experience. Also, understanding and handling mass spectrometry data is a multifaceted task that will require many decisions to be made to get the most comprehensive information from an experiment. In this mass Spectrometry-Based Proteomics Analysis Certification Course, we, therefore, aim to provide an overview of state-of-art techniques for the analysis & characterization of protein and peptide samples by mass spectrometry (MS/MS).
This course will surely help in understanding in-depth basics of mass spectrometry, sample preparation for analysis in mass spectrometer, type of instrumentation, fragmentation method, quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry, analysis strategy best suited to an individual sample as this technique is mostly used in proteomics-based research nowadays. Also, how mass spectrometer can be used to accurately distinguish different isoforms or modified forms of proteins (Post-translational modification), even in mixtures, that can provide added dimensions in the design of quantitative strategies and simplifies multiplex assay development.
- Course content meets the industry standard
- You can attend the course online anywhere anytime
- Interactive sessions with on spot doubt clearance
- A hard copy of Certificates will be provided on the course completion that adds weightage to your profile.
- You are getting lifetime access to this course
- Course sessions are taken by Senior Faculties of Biotecnika, Dr. Priyanka Sen and Dr. Tanushree Saxena.
The Mass Spectrometry-Based Proteomics Analysis Certification Course content is as follows:
- Proteomics introduction and sample preparation, in-gel & in-solution digestion, fundamentals of mass spectrometry
- Basics of mass spectrometry (Ionization sources, Mass analyzers, MALDI sample preparation and analysis, MS/ MS fragmentation of peptides, e.g.CID, HCD & ETD)
- Quantitative proteomics (Hybrid mass spectrometry configurations, SILAC, iTRAQ, TMT)
- Analysis of post-translational modifications by MS, including phosphopeptide enrichment & sequencing techniques.
- Proteomics and systems biology (Quantitative proteomics data analysis, Challenges in proteomics).
Eligibility for the Mass Spectrometry-Based Proteomics Analysis Certification Course:
All Life Science graduates, postgraduates, teaching & industry professionals along with research enthusiasts can apply. A basic level of knowledge about various concepts involved in life science is required. Good internet speed & a laptop are required for live streaming.
- What is an Online Self Learning Course?
An Online Self Learning Certification Course means you can log in any time and study at your own pace. However, you must watch 90% of all the lessons to proceed to the next lesson and if you want a Hard copy certificate you must clear the evaluation test, which will be available at the end of the course.
- How to get started with the Mass Spectrometry-Based Proteomics Analysis self-learning course?
Once you enroll for the awesome course, You will get the activation email almost immediately. start and finish the course at your own pace! Start learning whenever you can. There is no need to bend your schedule.
- How to unlock the next lesson of the course?
Once you master a session hit the Complete and Continue button on the top to begin your next session. Make sure you don't skip through! Unless you complete the current lesson you cannot proceed to the next lesson
- What is the time limit to complete the course?
Unlimited access until you decide to complete the course. But once you have given your certification test, the course ends. If you fail in the test, you can revise the course again
- How do I evaluate myself?
Time to test all the knowledge you have gained. Take the test at the end of the Mass Spectrometry-Based Proteomics Analysis course and evaluate yourself. Every Self Learning Course has a test at the end.
- How to get the hard copy certificate?
To avail, the hard copy certificate, Take the test at the end of the course. Clearing the test ( with a minimum of 40%) will earn you the certificate. The certificate will be sent to your doorstep. Now you know not to skip through the course.
- How long can I access the course?
You are getting lifetime access to the Mass Spectrometry-Based Proteomics Analysis course.
- Whom do I contact for any further queries?
- Will BioTecNika Provide any Job Guarantee / Assistance?
No, BioTecNika does not provide any Job Assistance or Guarantee. However you are free to apply for a Job on BioTecNika’s Job Portal and certainly, employers will give more weightage to you due to the reputation of BioTecNika and the Knowledge possessed by you after the course. The Mass Spectrometry-Based Proteomics Analysis Certification Course from BioTecNika is well respected and honored by most of the employers in the industry
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