Program 11: Diploma in Pharma Biotechnology
Pharma Biotechnology has detailed emphasis on fundamentals as well as advanced methodologies. The program involves main aspects of core subjects of microbiology, biochemistry & molecular biology which become the most important while entering industry.
Course Content:
Module 1:
Pharmaceutical Microbiology:
- Introduction to Microbiology Laboratory
- Isolation of Microorganisms
- Pure culture preparation and preservation techniques
-
Genus level identification of unknown microorganisms
- Culture characteristic studies
- Staining techniques
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- Simple staining technique
- Differential staining technique
- Viability staining technique for bacteria
-
Biochemical studies
- Differentiation of enteric microorganisms
- Extracelluar enzymatic activities of microorganisms
- Detection of Coli forms using membrane filtration method
- Isolation and identification of fungi form soil sample
- Isolation and identification of Actinomycetes and Mycoplasma
- Siderophore detection
- Phage isolation
- Bacteriological examination of water by Multiple Tube Fermentation test (MPN test)
- Production and detection of Aflatoxin
- Mutational studies and Replica plating technique
- Biotransformation technique
- Biodegradation technique
- Production of an Antibiotic (Aerobic fermentation)
- Microbiological assay of antibiotics by agar diffusion method
- Microbiological assay of antibiotics by Turbidimetric method
- Ames test for detecting potential carcinogens
- Detection of nicotinic acid by bioassay
- Detection of antibiotics by Bioautography technique
- Determination of MIC of antibacterial substances (phenol)
- Heparin bio-assay
- Screening Methods for bioactive metabolites
- Microbiological analysis of vitamins
-
Preparation of Bio molecules
- Preparation of plant DNA by SDS method and c-TAB method
- Preparation of animal DNA
- Preparation of RNA
- Preparation of Plasmid DNA
- Preparation of genomic DNA from prokaryotes
- Quantitative and Qualitative analysis of DNA
- Bacterial Genetics for Transformation
- Characterization of nutritional mutants
- Characterization of antibiotic resistance
- Characterization of UV sensitive mutants
- Preparation of competent coli cells (ultra competent)
- Expression of cloned genes in coli
- Gene regulation
- Gene expression and identification by western blotting
- Determination of Biomolecule
- Tm of DNA-Melting point of DNA
- Agarose gel Electrophoresis
- Polyacrylamide Electrophoresis
- SDS-PAGE
- Native-PAGE
- Urease-PAGE
- Recovery of DNA from Agarose
- Protein structure analysis
- Biodiversity and Biomass analysis
- Molecular Characterization
- PCR Techniques
- Southern Blotting
- Restriction Mapping
- Primer design and vector construction
- DNA Finger printing (by RAPD)
- Protein finger printing/protein profiling
MODULE 3: INSTRUMENTATION ANALYSIS
- HPLC
- GC
- AAS
- PCR
MODULE 4: IMMUNOLOGICAL TECHNIQUES
Part-I
- Introduction to immunology
- Raising polyclonal antibody in laboratory animal
- Selection of animal for antibody production
- Preparation of antigen
- Immunization of antigen
- Collection of Blood from immunized animal
- Serum separation by centrifugation
- Separation of serum by native PAGE
- Erythrocyte Sedimentation Rate (ESR)
- Purification of IgG from serum sample
- Salt precipitation test
- Dialysis
- Ion exchange chromatography
Part II
- Spectrophotometric estimation
- Quantitative estimation of proteins
- SDS PAGE
- Separation methods
- Separation of lymphocytes
- Serum electrophoresis for fractionization
- Gel diffusion methods
- Ouchterlony double diffusion
- Single radial imuno diffusion
- Immunoelectrophorosis
- Countercurrent Immunoelectrophorosis
- Rocket Immunoelectrophorosis
- Agglutination methods
- Blood grouping and Rh typing
- Fibril agglutination test (Widal test)
- VDRL/RPR test
- Hbs Ag test
Part III
- Immunochemistry methods
- ELISA
- Pregnancy test
- Estimations:
- Estimation of Blood hemoglobin using Haemometer
- Prothrombin time & Partial thromboplastin time determination
- Anti A1 lectin test
- Direct & indirect Antiglobulin Test (Coomb’s test)
- Packed Cell Volume (PCV)
- Measurement of serum albumin
- Separation of serum amino acids by paper chromatography
- Blood smear preparation, Staining & visualization of blood cells
- Coagulation method fibrinogen
Who should join?
If you are a biotechnology enthusiast, an MSc/MTech/ME in Life Sciences, who are looking to strengthen concepts and build on hands-on skills, which would help you grab the right job, this is the right place to enrol.
Course Details:
The module consists of 90 days classroom training with our expert faculty followed by 30 days of practical exposure with our service provider. Also avail our e-learner facility.
Total Duration: 120 days
The classes will be held in Biotecnika Bangalore branch/ Online.
Lab Partner: Azyme labs