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Program 11: Diploma in Pharma Biotechnology

  • Rs 45,00000


Pharma Biotechnology has detailed emphasis on fundamentals as well as advanced methodologies. The program involves main aspects of  core subjects of microbiology, biochemistry & molecular biology which become the most important while entering industry.

Course Content:

Module 1:

Pharmaceutical Microbiology:

  • Introduction to Microbiology Laboratory
  • Isolation of Microorganisms
  • Pure culture preparation and preservation techniques
  • Genus level identification of unknown microorganisms
    • Culture characteristic studies
    • Staining techniques
      • Simple staining technique
      • Differential staining technique
      • Viability staining technique for bacteria
    • Biochemical studies
      • Differentiation of enteric microorganisms
      • Extracelluar enzymatic activities of microorganisms
  • Detection of Coli forms using membrane filtration method
  • Isolation and identification of fungi form soil sample
  • Isolation and identification of Actinomycetes and Mycoplasma
  • Siderophore detection
  • Phage isolation
  • Bacteriological examination of water by Multiple Tube Fermentation test (MPN test)
  • Production and detection of Aflatoxin
  • Mutational studies and Replica plating technique
  • Biotransformation technique
  • Biodegradation technique
  • Production of an Antibiotic (Aerobic fermentation)
  • Microbiological assay of antibiotics by agar diffusion method
  • Microbiological assay of antibiotics by Turbidimetric method
  • Ames test for detecting potential carcinogens
  • Detection of nicotinic acid by bioassay
  • Detection of antibiotics by Bioautography technique
  • Determination of MIC of antibacterial substances (phenol)
  • Heparin bio-assay
  • Screening Methods for bioactive metabolites
  • Microbiological analysis of vitamins
MODULE 2: RECOMBINANT DNA TECHNOLOGY
  • Preparation of Bio molecules

    • Preparation of plant DNA by SDS method and c-TAB method
    • Preparation of animal DNA
    • Preparation of RNA
    • Preparation of Plasmid DNA
    • Preparation of genomic DNA from prokaryotes
    • Quantitative and Qualitative analysis of DNA
  • Bacterial Genetics for Transformation
    • Characterization of nutritional mutants
    • Characterization of antibiotic resistance
    • Characterization of UV sensitive mutants
    • Preparation of competent coli cells (ultra competent)
    • Expression of cloned genes in coli
    • Gene regulation
    • Gene expression and identification by western blotting
  • Determination of Biomolecule
    • Tm of DNA-Melting point of DNA
    • Agarose gel Electrophoresis
    • Polyacrylamide Electrophoresis
      • SDS-PAGE
      • Native-PAGE
      • Urease-PAGE
    • Recovery of DNA from Agarose
    • Protein structure analysis
    • Biodiversity and Biomass analysis
  • Molecular Characterization
    • PCR Techniques
    • Southern Blotting
    • Restriction Mapping
    • Primer design and vector construction
    • DNA Finger printing (by RAPD)
    • Protein finger printing/protein profiling

MODULE 3: INSTRUMENTATION  ANALYSIS

  • HPLC
  • GC
  • AAS
  • PCR

    MODULE 4: IMMUNOLOGICAL TECHNIQUES

    Part-I

    • Introduction to immunology
    • Raising polyclonal antibody in laboratory animal
      • Selection of animal for antibody production
      • Preparation of antigen
      • Immunization of antigen
      • Collection of Blood from immunized animal
      • Serum separation by centrifugation
      • Separation of serum by native PAGE
      • Erythrocyte Sedimentation Rate (ESR)
    • Purification of IgG from serum sample
      • Salt precipitation test
      • Dialysis
      • Ion exchange chromatography

    Part II

    • Spectrophotometric estimation
    • Quantitative estimation of proteins
    • SDS PAGE
    • Separation methods
      • Separation of lymphocytes
      • Serum electrophoresis for fractionization
    • Gel diffusion methods
      • Ouchterlony double diffusion
      • Single radial imuno diffusion
      • Immunoelectrophorosis
      • Countercurrent Immunoelectrophorosis
      • Rocket Immunoelectrophorosis
    • Agglutination methods
      • Blood grouping and Rh typing
      • Fibril agglutination test (Widal test)
      • VDRL/RPR test
      • Hbs Ag test

    Part III

    • Immunochemistry methods
      • ELISA
      • Pregnancy test
    • Estimations:
      • Estimation of Blood hemoglobin using Haemometer
      • Prothrombin time & Partial thromboplastin time determination
      • Anti A1 lectin test
      • Direct & indirect Antiglobulin Test (Coomb’s test)
      • Packed Cell Volume (PCV)
      • Measurement of serum albumin
      • Separation of serum amino acids by paper chromatography
      • Blood smear preparation, Staining & visualization of blood cells
      • Coagulation method fibrinogen

    Who should join?

    If you are a biotechnology enthusiast, an MSc/MTech/ME in Life Sciences, who are looking to strengthen concepts and build on hands-on skills, which would help you grab the right job, this is the right place to enrol. 

    Course Details:

    The module consists of 90 days classroom training with our expert faculty followed by 30 days of practical exposure with our service provider. Also avail our e-learner facility.

    Total Duration: 120 days

    The classes will be held in Biotecnika Bangalore branch/ Online. 

    Lab Partner: Azyme labs


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