Program 10: Diploma in General Biotechnology

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Rs. 45,000.00
Rs. 44,900.00

Get a gist of Industrial Biotechnology, Recombinant DNA technology, Instrumentation, Enzyme Biotechnology- all of which forms the basis of your working capabilities in a biotech/pharma laboratory. 

Course Content:

The Diploma Program consists of 4 Modules as detailed below:

MODULE 1: INDUSTRIAL BIOTECHNOLOGY

  • Sterilization techniques
  • Isolation of Industrially important Microorganisms from natural sources
  • Pure culture techniques
    • Streak plate technique
    • Spread plate technique
  • Genus level identification of unknown microorganisms
    • Culture characteristic studies
    • Staining techniques
    • Biochemical techniques
  • Bacteriological examination of water by Multiple Tube Fermentation test (MPN test)
  • Detection of Coli forms using membrane filtration method
  • Determination Bacterial Growth Curve by Spectrophotometric method
  • Microbiological analysis of food products
  • Screening of microorganisms for production of enzymes
    • Starch hydrolysis test
    • Casein hydrolysis test
    • Catalase test
    • Urea hydrolysis test
    • Gelatin hydrolysis test
  • Production of enzymes
  • Enzyme assay
  • Screening of microorganisms for production of Antibiotics
    • Isolation of antibiotic producing bacteria
    • Production of antibiotics
    • Antibiotic sensitivity test
  • Production and estimation of citric acid from fungi
  • Detection of arsenic by microbiological methods 
  • Detection of nicotinic acid by bioassay
  • Production of wine from grapes
  • Production of sauerkraut by microorganisms
  • Production of amino acids and detection by chromatographic method
  • Demonstration of Koch’s postulates for fungal pathogens
  • Screening for antagonism
  • Production, purification and characterization of Enzymes/Amino acids /Organic acid/Antibiotics 

MODULE 2: RECOMBINANT DNA TECHNOLOGY

Part I:

 Preparation of plant DNA by SDS method and c-TAB method

  • Preparation of RNA
  • Preparation of animal DNA
  • Preparation of Plasmid DNA
  • Preparation of genomic DNA from prokaryotes
  • Quantitative and Qualitative analysis of DNA
  • Characterization of bacterial strains
    • Characterization of nutritional mutants
    • Characterization of antibiotic resistance
    • Characterization of UV sensitive mutants

Part II:

  • Tm of DNA-Melting point of DNA
  • Restriction mapping
  • Preparation of competent coli cells (ultra competent)
  • Expression of cloned genes in coli
  • Gene regulation
  • PAGE and AGE for DNA
  • Protein structure analysis
  • Biodiversity and Biomass analysis
  • Recovery of DNA from Agarose.
  • Detection of DNA
    • Silver staining

Part III:

  • Southern Blotting
  • Gene expression and identification by western blotting
  • Primer design and vector construction
  • PCR Techniques
  • DNA Finger printing (by RAPD)
  • Protein finger printing/protein profiling

MODULE 3: INSTRUMENTATION

  • HPLC
  • GC
  • AAS
  • PCR

MODULE 4: ENZYME BIOTECHNOLOGY

  • Sterilization techniques
  • Isolation of enzyme producing microorganisms from natural sources
  • Pure culture preparation and preservation techniques
  • Genus level identification of unknown microorganisms
    • Staining techniques
    • Biochemical studies
  • Screening for production of enzymes
  • Fermentation technique
    • Solid state fermentation technique
    • Submerged fermentation techniques
  • Down stream processes
    • Crude extraction of enzyme
    • Purification of crude enzyme
    1. Precipitation of enzymes (salt/solvent)
    2. Dialysis
    3. Ion exchange chromatography
    4. Gel filtration chromatography
  • Characterization of purified enzyme
  • Enzyme assay
    • Total protein estimation
  1. Bradford method
  2. Biuret method
  3. Lowry’s method
  4. 280 methods
  • Concept of specific activity
  • Enzyme kinetics
  • Effect of pH
  • Effect of Temperature
  • Effect of Substrate conc.
  • Effect of Activator
  • Effect of Inhibitor
  • Time course of the reaction
  • The concept of Km and Vamp
  • Demonstration of substrate catalyst complex
  • SDS PAGE
  • Staining of proteins
  • CBB staining
  • Silver staining
  • Drying gels
  • Calibration of proteins and molecular weight determination
  • Native PAGE
  • Zymography
  • Immobilization of Enzymes and formulation

Who should join?

If you are a biotechnology enthusiast, an MSc/MTech/ME in Life Sciences, who are looking to strengthen concepts and build on hands-on skills, which would help you grab the right job, this is the right place to enrol. 

Course Details:

The module consists of 3 month classroom training with our expert faculty followed by 1 months practical exposure with our service provider. Also avail our e-learner facility.

Total Duration: 4 months

The classes will be held in Biotecnika Bangalore branch/ Online. 

Lab Partner: Azyme labs

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